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KMID : 0376219830200010107
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Chonnam Medical Journal
1983 Volume.20 No. 1 p.107 ~ p.114
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Phage hemagglutination-forming ability of the Klebsiella pneumoniae resistant to streptomycin
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朴寶漢/Park, Bohan
安泰烋/李炫哲/辛富安/Ahn, Tai-Hew/Lee, Hyun Chul/Shin, Boo Ahn
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Abstract
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The author has studied, using the Klebsiella phage-host cell system and sheep red cells, on the cold and warm phage hemagglutination(CPH and WPH)-forming ability of the Klebsiella pneumoniae (Kp) resistant to streptomycin (SM). The following results were obtained.
The minimum inhibitory dose of SM to Kp 4, Kp 5 and normal Kp used in this experiment was 0.625mg/ml, 160 mg/ml and 0.02mg/ml, respectively.
The CPH-and WPH-forming abilities of Kp 4 were similar to that of Kp. However, the CPH-and WPH-forming abilities of Kp 5 were a little stronger than that of Kp. The phage adsorption, at cold temperature, to Kp 5 substance-sensitized red cells was a little faster and higher grade than that to the Kp or Kp 4 substance-sensitized red cells.
The phage inactivating activity, at warm temperature, of Kp 5 substance, against Kp 4 substance, was a little stronger than that of Kp substance.
The phage elution from Kp or Kp 5 substance-sensitized red cells was similar.
The hemagglutination reaction by Kp 5, against Kp 4, substance-sensitized red cells and anti-Kp serum was a little weaker than that occurred by Kp substance-sensitized red cells.
When the Kp substance-sensitized red cell is used, the anti-Kp serum absorbed with KP 5 substance produced still a little hemagglutination reaction. The antiserum, however, absorbed with Kp 4 substance did not.
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